ZDHHC8 Antibody

SKU: orb19438

Description

Goat polyclonal antibody to ZDHHC8

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:16000, WB: 1-3 μg/ml
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Porcine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 80-85kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 81.4kDa according to NP_037505.1). Recommended concentration: 1-3 μg/ml. An additional band of 48kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: ZDHHC8
• Protein Sequence: QRDHPQLKTPPSK
• Molecular Weight: 81.4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti ZDHHC8 antibody, anti zinc finger, DHHC-type containing 8 antibody, anti ZDHHCL1 antibody, anti ZNF378 antibody, anti membrane-associated DHHC8 zinc finger protein antibody, anti zinc finger, DHHC domain containing 8 antibody, anti zinc finger, DHHC domain like containing 1 antibody

orb19438 (0.1ug/ml) staining of Human Brain (Frontal Cortex) lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

ZDHHC8

NCBI Gene ID

29801