Zcchc11 Antibody

• Catalog Number: orb1248826
• Category: Antibodies
• Description: Zcchc11 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Mouse, Rat
• Immunogen: The immunogen for this antibody is: C-QKSAEKQGDLTPSET
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 170kDa band observed in Rat Brain lysates (calculated MW of 163kDa according to Rat NP_001101423.1 and 185kDa according to Mouse NP_780681.2). Recommended concentration: 0.03-0.1ug/ml. Approx 200kDa band observed in Mouse Testis lysates and not in Knock-out background (calculated MW of 185kDa according to Human NP_001009881.1 and to Mouse NP_780681.2). Recommended concentration: 0.1-0.3ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 170 kDa
• Target: Zcchc11
• UniProt ID: B2RX14
• NCBI: NP_780681.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: Zcchc11, zinc finger, CCHC domain containing 11, R
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 170kDa band observed in Rat Brain lysates (calculated MW of 163kDa according to Rat NP_001101423.1 and 185kDa according to Mouse NP_780681.2). Recommended concentration: 0.03-0.1ug/ml. Approx 200kDa band observed in Mouse Testis lysates and not in Knock-out background (calculated MW of 185kDa according to Human NP_001009881.1 and to Mouse NP_780681.2). Recommended concentration: 0.1-0.3ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248826 (0.05 ug/ml) staining of Rat Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248826 (0.2 ug/ml) staining of different Testis lysates from wildtype 8-9 week old C57BL/6 (wt) and knock-out (KO) mice (35 ug protein in RIPA buffer). Primary incubation was 2 hours. Detected by chemiluminescence. Data obtained from an anonymous customer.