Mirin

SKU: orb1304504

Description

(Z)-Mirin

Research Area

Cell Biology, Molecular Biology, Signal Transduction

Key Properties

• CAS Number: 1198097-97-0
• MW: 220.3
• Purity: >98%
• Formula: C₁₀H₈N₂O₂S
• SMILES: Oc1ccc(\C=C2/SC(=N)NC2=O)cc1
• Target: DNA
• Solubility: Soluble in DMSO (up to 30 mg/ml).

Bioactivity

• In Vitro:
  Mirin inhibits DSB-induced ATM activation, the ATM-dependent phosphorylation of the downstream targets Nbs1 and Chk2 and the MRN-dependent autophosphorylation of ATM at Ser1981 in response to DSBs. Mirin also inhibits the G2 checkpoint in TOSA4 cells, and homology-dependent DNA repair in HEK293 cells. In cells with integrated HPV16 (SiHa), Mirin sensitizes HPV episomes to PA25 resulting in a ～5-fold reduction of the PA25 IC50. Pretreatment with mirin also decreases cell viability and inhibits proliferating cell nuclear antigen expression in cisplatin-treated human embryonic kidney 293 cells.
• Cell Research:
  Human embryonic kidney (HEK) 293 cells are maintained in RPMI-1640 supplemented with 5% heat-inactivated fetal bovine serum, penicillin (100 U/mL), and streptomycin (100 mg/mL) in humidified air with 5% CO2 at 37 °C. Cells are given fresh medium at 48 h intervals. The cells are seeded in 96-well plates in regular growth medium. Cells are pretreated with mirin (100 μM for 1 h before the cisplatin (20 μM) treatment followed by incubation for 8 and 24 h. The MTT assay is performed using the EZ-Cytox cell viability assay kit according to the manufacturer's protocol and MTT reduction is measured at a 450 nm wavelength using a micro-plate reader. (Only for Reference)

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

(Z)Mirin, (Z)-Mirin, (Z) Mirin, ATM, ATR, Mirin, MRN

Compound Identifiers

PubChem CID

137319711