PFM01

SKU: orb1298556

Description

PFM01 is a small molecule inhibitor of the MRE11 endonuclease, enabling researchers to modulate the cellular DNA double-strand break repair pathway choice. It suppresses homologous recombination (HR) and can promote error-prone non-homologous end joining (NHEJ), making it a valuable tool for studying DNA repair mechanisms in cancer research and cellular response to genotoxic agents both in vitro and in vivo.

Research Area

Cell Biology

Key Properties

• CAS Number: 1558598-41-6
• MW: 293.4
• Purity: 98.68% (May vary between batches)
• Formula: C₁₄H₁₅NO₂S₂
• SMILES: CC(C)CN1C(=S)S\C(=C/c2ccc(O)cc2)C1=O
• Target: Endonuclease,Others
• Solubility: DMSO:4 mg/mL (13.63 mM)

Bioactivity

• In Vitro:
  PFM01 (100 μM) treatment allows normal DSB repair in HR-defective ATMi-treated or HSC62 (BRCA2-defective) cells. RAD51 foci did not form following exo- or endo-inhibitor treatment (mirin or PFM01) in 1BR3 (WT) and HSC62 (BRCA2-defective) cells. PFM01 (100 μM) enhances non-homologous end-joining (NHEJ) in H1299 dA3 cells and reduces homologous recombination (HR) in U2OS DR-GFP cells. PFM01 substantially relieves the double-strand break (DSB) repair defect confers by mirin or PFM39 in irradiated G2 cells.

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PFM 01, PFM01, PFM-01, endonuclease, inhibit, Inhibitor, MRE11, N-alkylated, NHEJ, Mirin, derivative, DSB

Compound Identifiers

PubChem CID

2145809