YTHDC1 Antibody

• Catalog Number: orb1256468
• Category: Antibodies
• Description: YTHDC1 Antibody
• Target: YTHDC1
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 260-500 of human YTHDC1 (NP_001026902.1).
• UniProt ID: Q96MU7
• MW: Observed: 118kDa
• Tested applications: IF, WB
• Application notes: WB: 1:500 - 1:2000IF: 1:50 - 1:100
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: YT521, YT521-B, YTH domain-containing protein 1, p
• Note: For research use only

Western blot analysis of extracts of various cell lines, using YTHDC1 antibody (orb1256468) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Immunofluorescence analysis of A-549 cells using YTHDC1 antibody (orb1256468). Blue: DAPI for nuclear staining.