YBX1 Antibody

SKU: orb1259382

Description

YBX1 Antibody

Research Area

Cell Biology, Epigenetics & Chromatin

Images & Validation

• Tested Applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human YBX1 (NP_004550.2).
• Target: YBX1
• Molecular Weight: Observed: 49kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NSEP1, YB1, BP-8, CSDB, DBPB, YB-1, CSDA2, NSEP1, NSEP-1, MDR-NF1

Western blot analysis of extracts of HepG2 cells, using YBX1 antibody (orb1259382) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunofluorescence analysis of U2OS cells using YBX1 antibody (orb1259382) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using YBX1 antibody (orb1259382) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using YBX1 antibody (orb1259382) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using YBX1 antibody (orb1259382) at dilution of 1:100. Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

YBX1

UniProt

P67809