YBX1 Antibody, KO Validated

• Catalog Number: orb1274422
• Category: Antibodies
• Description: YBX1 Antibody, KO Validated
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human YBX1 (NP_004550.2).
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 49kDa
• Target: YBX1
• UniProt ID: P67809
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: NSEP1, YB1, BP-8, CSDB, DBPB, YB-1, CSDA2, NSEP1,
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:100 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of HepG2 cells, using YBX1 antibody (orb1274422) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Western blot analysis of extracts from normal (control) and YBX1 knockout (KO) HeLa cells, using YBX1 antibody (orb1274422) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Immunohistochemistry of paraffin-embedded human lung cancer using YBX1 antibody (orb1274422) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human prostate using YBX1 antibody (orb1274422) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using YBX1 antibody (orb1274422) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using YBX1 antibody (orb1274422) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using YBX1 antibody (orb1274422) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using YBX1 antibody (orb1274422) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using YBX1 antibody (orb1274422) at dilution of 1:100. Blue: DAPI for nuclear staining.