Y15

SKU: orb1300054

Description

Y15 (1,2,4,5-Benzenetetramine tetrahydrochloride) is a specific small-molecule inhibitor targeting FAK scaffolding to block its autophosphorylation. It has demonstrated efficacy in suppressing tumor growth in both cellular assays and animal models, making it a valuable tool for cancer research.

Research Area

Cardiovascular Research, Signal Transduction

Key Properties

• CAS Number: 4506-66-5
• MW: 284.01
• Purity: 99.32% (May vary between batches)
• Formula: C₆H₁₄Cl₄N₄
• SMILES: Cl.Cl.Cl.Cl.Nc1cc(N)c(N)cc1N
• Target: FAK
• Solubility: H2O:59 mg/mL (207.74 mM)

Bioactivity

• In Vitro:
  Y15 inhibited viability in thyroid cancer cell lines in a dose-dependent manner.
• Cell Research:
  Apoptosis assay was done with PE Annexin V Apoptosis Detection Kit (BD) to identify apoptotic nuclei. One hundred millimeter plates were seeded with TPC1, K1, BCPAP and TT cells at a concentration of 1 × 10^6 cells in normal growth media. After overnight incubation, plates were treated with one of several doses of Y15 or left untreated. After another 24 h of incubation, cells were collected with trypsin, and following centrifugation, were washed with 1× PBS. Again, cells were centrifuged, PBS was removed and cells were resuspended in binding buffer and filtered through BD mesh tubes. Annexin V and 7-AAD were added to the solution, vortexed and incubated for 15 minutes in the dark. Binding buffer was added and the cells were analyzed by flow cytometry within 1 hour.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

inhibit, FAK, FAK Inhibitor 14, FAK inhibitor Y15, FAK Inhibitor14, FAK Inhibitor-14, Focal adhesion kinase, Inhibitor, 1,2,4,5-Benzenetetramine tetrahydrochlor, PTK2, PTK2 protein tyrosine kinase 2, Y 15, Y15, Y-15

Compound Identifiers

PubChem CID

78260