XPO5 Antibody

• Catalog Number: orb1256772
• Category: Antibodies
• Description: XPO5 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 925-1204 of human XPO5 (NP_065801.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 125kDa
• Target: XPO5
• UniProt ID: Q9HAV4
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: Exportin-5, Exp5, Ran-binding protein 21, XPO5, KI
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using XPO5 antibody (orb1256772). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human oophoroma using XPO5 antibody (orb1256772) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse jejunum using XPO5 antibody (orb1256772) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of MCF7 cells using XPO5 antibody (orb1256772).

Immunoprecipitation analysis of 200 ug extracts of HeLa cells using 1 ug XPO5 antibody (orb1256772). Western blot was performed from the immunoprecipitate using XPO5 antibody (orb1256772) at a dilution of 1:1000.