XPNPEP1 Antibody

• Catalog Number: orb1249611
• Category: Antibodies
• Description: XPNPEP1 Antibody
• Target: XPNPEP1
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Porcine, Rat
• Predicted Reactivity: Bovine, Canine
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-LIRETQPISKQH
• UniProt ID: Q9NQW7
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 80kDa band observed in Human Heart, Skeletal Muscle and Pancreas lysates and 75kDa band observed in Mouse and Rat Small Intestines, and 75kDa band observed in Pig Pancreas lysate (calculated MW of 69.9kDa according to NP_065116.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Pancreas. Recommended concentration: 5ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: XPNPEP1, X-prolyl aminopeptidase (aminopeptidase P
• Research Area: Signal Transduction
• Note: For research use only
• NCBI: NP_001161076.1, NP_065116.2

orb1249611 (0.3 ug/ml) staining of Human Pancreas lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249611 (0.1 ug/ml) staining of Mouse (A) and Rat (B) Small Intestine lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249611 (0.1 ug/ml) staining of Pig Pancreas lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249611 (5 ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.