XPNPEP1 Antibody

• Catalog Number: orb1249611
• Category: Antibodies
• Description: XPNPEP1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine
• Reactivity: Human, Mouse, Porcine, Rat
• Immunogen: The immunogen for this antibody is: C-LIRETQPISKQH
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 80kDa band observed in Human Heart, Skeletal Muscle and Pancreas lysates and 75kDa band observed in Mouse and Rat Small Intestines, and 75kDa band observed in Pig Pancreas lysate (calculated MW of 69.9kDa according to NP_065116.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Pancreas. Recommended concentration: 5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: XPNPEP1
• UniProt ID: Q9NQW7
• NCBI: NP_001161076.1, NP_065116.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: XPNPEP1, X-prolyl aminopeptidase (aminopeptidase P
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 80kDa band observed in Human Heart, Skeletal Muscle and Pancreas lysates and 75kDa band observed in Mouse and Rat Small Intestines, and 75kDa band observed in Pig Pancreas lysate (calculated MW of 69.9kDa according to NP_065116.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Pancreas. Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1249611 (0.3 ug/ml) staining of Human Pancreas lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249611 (0.1 ug/ml) staining of Mouse (A) and Rat (B) Small Intestine lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249611 (0.1 ug/ml) staining of Pig Pancreas lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249611 (5 ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.