Anti-XPNPEP1 [SAIC-07E-1]

• Catalog Number: orb1089998
• Category: Antibodies
• Description: Rabbit monoclonal antibody to XPNPEP1
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: SAIC-07E-1
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Peptide "TLSLDEVYLIDSGAQYK" derived from Xaa-Pro aminopeptidase 1 conjugated to KLH.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: XPNPEP1
• UniProt ID: Q9NQW7
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Xaa-Pro aminopeptidase 1; XPNPEPL; XPNPEPL1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the Anti-XPNPEP1 antibody SAIC-07E-1. Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of SAIC-07E-1 (orb1089998, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of HeLa cells with anti-XPNPEP1 SAIC-07E-1. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-07E-1 (orb1089998) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1089998, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using anti-XPNPEP1 antibody SAIC-07E-1. HeLa(A) (0.003 µg/ml), Jurkat(B) (0.001 µg/ml), MCF7(C) (0.001 µg/ml), HepG2(D) (0.003 µg/ml), A549(E) (0.001 µg/ml) and HEK293(F) (0.003 µg/ml) cell lysates (35 µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-07E-1 (orb1089998), before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.