WNT5 Antibody

• Catalog Number: orb1238587
• Category: Antibodies
• Description: WNT5 Antibody
• Target: WNT5B
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Rabbit
• Form/Appearance: Liquid
• Concentration: 1 mg/mL
• Buffer/Preservatives: WNT5 antibody is supplied in PBS containing 0.02% sodium azide.
• Purification: WNT5 antibody is affinity chromatography purified via peptide column.
• Immunogen: WNT5 antibody was raised against a 15 amino acid peptide near the center of human WNT5B.The immunogen is located within amino acids 130 - 180 of WNT5.
• UniProt ID: Q9H1J7
• MW: Predicted: 39 kDa Observed: 465 kDa
• Tested applications: ELISA, IHC-P, WB
• Application notes: WNT5 antibody can be used for detection of WNT5 by Western blot at 1 - 2 μg/ml. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Wingless-type MMTV integration site family member
• Research Area: Cancer Biology, Cell Biology
• Note: For research use only
• NCBI: NP_110402

Western blot analysis of WNT5 in Jurkat cell lysate with WNT5 antibody at 1 µg/ml in (A) the absence and (B) the presence of blocking peptide.

Immunohistochemistry of WNT5 in rat colon tissue with WNT5 antibody at 5 µg/mL.