WNT5B Antibody (Center)

SKU: orb1928404

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:10-50, FC - 1:10-50
• Reactivity: Human, Mouse
• Predicted Reactivity: Other, Rabbit, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB22511
• Immunogen: This WNT5B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 153-182 amino acids from the Central region of human WNT5B. Antigen Region: 153-182 aa.
• Target: WNT5B
• Molecular Weight: 40323 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Protein Wnt-5b, WNT5B

Western blot analysis of WNT5B Antibody (Center) in mouse bladder tissue lysates (35 ug/lane). WNT5B (arrow) was detected using the purified Pab.

Flow cytometric analysis of widr cells using WNT5B Antibody (Center) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human skeletal muscle with WNT5B Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Quick Database Links

Gene Symbol

WNT5B

UniProt

Q9H1J7

RefSeq (Protein):NP_110402.2, NP_116031.1