WNT5B Antibody (Center)

• Catalog Number: orb1928404
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB22511
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Other, Rabbit, Rat
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 40323 Da
• Target: This WNT5B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 153-182 amino acids from the Central region of human WNT5B.
• UniProt ID: Q9H1J7
• NCBI: NP_110402.2, NP_116031.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Protein Wnt-5b, WNT5B
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of WNT5B Antibody (Center) in mouse bladder tissue lysates (35 ug/lane). WNT5B (arrow) was detected using the purified Pab.

Flow cytometric analysis of widr cells using WNT5B Antibody (Center) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human skeletal muscle with WNT5B Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.