WNT3A Antibody

• Catalog Number: orb1539118
• Category: Antibodies
• Description: WNT3A Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, IHC-P, WB
• Reactivity: Human
• Immunogen: E.coli derived recombinant Human WNT-3a.
• Dilution range: ELISA (0.5 - 2 µg/ml), IHC, IHC-P (5 µg/ml), WB (0.1 - 0.2 µg/ml)
• Conjugation: Unconjugated
• Target: WNT3A
• Storage: Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Lyophilized from PBS, pH 7.2. Lyophilized from PBS, pH 7.2
• Alternative names: WNT3A, Protein Wnt-3a
• Note: For research use only
• Application notes: Resuspension: Reconstitute with sterile water to 0.1-1.0 mg/ml.. Further information: Immunohistochemistry: LS-B8210 in an antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. ELISA: To detect hWNT-3a by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human WNT-3a (LS-C104698) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hWNT-3a. Western Blot: To detect hWNT-3a by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hWNT-3a is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
• Expiration Date: 12 months from date of receipt.