Wnt3a Rabbit Polyclonal Antibody

SKU: orb584128

Description

Rabbit polyclonal antibody to Wnt3a

Images & Validation

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Item 1 of 4

Tested Applications	IHC, WB
Reactivity	Human, Mouse
Predicted Reactivity	Bovine, Canine, Equine, Guinea pig, Porcine, Rabbit, Rat, Zebrafish

Related Conjugates & Formulations

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Biotin

FITC

HRP

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	The immunogen is a synthetic peptide corresponding to a region of Mouse
Target	Wnt3a
Molecular Weight	38kDa
Purification	Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration	0.5 mg/ml
Disclaimer	For research use only

Alternative Names

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vt, Wnt-, Wnt-3a

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Sample Type: Mouse Heart, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 1 ug/ml, Peptide Concentration: 5 ug/ml, Lysate Quantity: 25 ug/lane, Gel Concentration: 0.12%.

Immunohistochemistry with Placenta tissue at an antibody concentration of dilution: 1:500 using anti-Wnt3a antibody (orb584128).

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/ml in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

WB Suggested Anti-Wnt3a Antibody Titration: 1 ug/ml, Positive Control: Mouse Heart lysate.

Quick Database Links

Gene Symbol

Wnt3a

UniProt

P27467

RefSeq (Protein):NP_033548

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_033548

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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