WNT3A Antibody

SKU: orb1272718

Description

WNT3A Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human
• Application Notes:
  ELISA:Indirect:To detect hWNT-3a by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hWNT-3a.SandwichTo detect hWNT-3a by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human WNT-3a as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hWNT-3a. Western Blot:To detect hWNT-3a by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hWNT-3a is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hWNT-3a. Human WNT-3a specific antibody was purified by affinity chromatography employing immobilized hWNT-3a matrix.
• Target: WNT3A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

Protein Wnt-3a

This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.125 ug/ml - 0.25 ug/ml with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.

Quick Database Links

Gene Symbol

WNT3A

UniProt

P56704

RefSeq:P56704