WDR82 Antibody (N-term)

SKU: orb1926798

Description

Purified Rabbit Polyclonal Antibody (Pab)

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:2000, IHC-P-Leica - 1:500
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD73646
• Immunogen: This WDR82 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 12~46 amino acids from the N-terminal region of human WDR82. Antigen Region: 12-46 aa.
• Target: WDR82 {ECO:0000303|PubMed:17998332, ECO:0000312|HGNC:HGNC:28826}
• Molecular Weight: 35079 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

WD repeat-containing protein 82, Protein TMEM113, Swd2, WDR82, TMEM113, WDR82A

All lanes: Anti-WDR82 Antibody (N-term) at 1:2000 dilution. Lane 1: NCCIT whole cell lysate. Lane 2: SH-SY5Y whole cell lysate. Lane 3: HCT116 whole cell lysate. Lane 4: U-251 MG whole cell lysate. Lane 5: NIH/3T3 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 35 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded human brain tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Immunohistochemical analysis of paraffin-embedded human placenta tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Quick Database Links

Gene Symbol

WDR82 {ECO:0000303|PubMed:17998332, ECO:0000312|HGNC:HGNC:28826}

UniProt

Q6UXN9