WDR82 Antibody (N-term)

SKU: orb1926799

Description

Purified Rabbit Polyclonal Antibody (Pab)

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:250, FC - 1:25
• Reactivity: Human, Mouse, Zebrafish
• Predicted Reactivity: Other

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD73647
• Immunogen: This WDR82 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 50-85 amino acids from the N-terminal region of human WDR82. Antigen Region: 50-85 aa.
• Target: WDR82 {ECO:0000303|PubMed:17998332, ECO:0000312|HGNC:HGNC:28826}
• Molecular Weight: 35079 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

WD repeat-containing protein 82, Protein TMEM113, Swd2, WDR82, TMEM113, WDR82A

Staining WDR82 in human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody (1/250) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

All lanes: Anti-WDR82 Antibody (N-term) at 1:1000 dilution. Lane 1: F9 whole cell lysate. Lane 2: NIH/3T3 whole cell lysate. Lane 3: SH-SY5Y whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 35 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Overlay histogram showing HepG2 cells stained (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed (1583138) at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

Quick Database Links

Gene Symbol

WDR82 {ECO:0000303|PubMed:17998332, ECO:0000312|HGNC:HGNC:28826}

UniProt

Q6UXN9