WDR82 Antibody (N-term)

• Catalog Number: orb1926799
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This WDR82 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 50-85 amino acids from the N-terminal region of human WDR82.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Zebrafish
• Predicted Reactivity: Other
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q6UXN9
• MW: 35079 Da
• Tested applications: FC, IHC-P, WB
• Dilution range: WB: 1:1000, IHC-P: 1:250, FC: 1:25
• Antibody Type: Primary Antibody
• Clone Number: RB51425
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: WD repeat-containing protein 82, Protein TMEM113,
• Note: For research use only

Staining WDR82 in human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody (1/250) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

All lanes: Anti-WDR82 Antibody (N-term) at 1:1000 dilution. Lane 1: F9 whole cell lysate. Lane 2: NIH/3T3 whole cell lysate. Lane 3: SH-SY5Y whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 35 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Overlay histogram showing HepG2 cells stained (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed (1583138) at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.