VPS41 Antibody

• Catalog Number: orb1246788
• Category: Antibodies
• Description: VPS41 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-DHLKKDSQNKTLLK
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave bands at approx 48kDa and 28kDa in human duodenum, kidney and placenta lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 98.5kDa according to NP_055211.1 and 100kDa according to NP_542198.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).Immunohistochemistry:In paraffin embedded Human Kidney shows staining of collective tubules. Recommended concentration: 4-6ug/ml. Paraffin embedded Human Placenta and Prostate. Recommended concentration: 3.75ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: VPS41
• UniProt ID: P49754
• NCBI: NP_055211.2, NP_542198.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: VPS41, vacuolar protein sorting 41 (yeast) , HGNC:
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave bands at approx 48kDa and 28kDa in human duodenum, kidney and placenta lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 98.5kDa according to NP_055211.1 and 100kDa according to NP_542198.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).Immunohistochemistry:In paraffin embedded Human Kidney shows staining of collective tubules. Recommended concentration: 4-6ug/ml. Paraffin embedded Human Placenta and Prostate. Recommended concentration: 3.75ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1246788 (4 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. Similar results were obtained after antigen retrieval at pH9.

orb1246788 (3.75 ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1246788 (3.75 ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.