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VPS41 Antibody

SKU: orb19098

Description

Goat polyclonal antibody to VPS41

Images & Validation

Tested ApplicationsELISA, IHC
Dilution rangeELISA: 1:16000, WB: 1 μg/ml, IHC-P: 3.75 μg/ml
ReactivityHuman
Predicted ReactivityBovine, Canine, Mouse, Rat
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave bands at approx 48kDa and 28kDa in human duodenum, kidney and placenta lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 98.5kDa according to NP_055211.1 and 100kDa according to NP_542198.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

HostGoat
ClonalityPolyclonal
TargetVPS41
Molecular Weight98.5; 95.9
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
DisclaimerFor research use only

Alternative Names

anti VPS41 antibody, anti vacuolar protein sorting 41 (yeast) antibody, anti HGNC:12713 antibody, anti HVSP41 antibody, anti vacuolar assembly protein 41 antibody, anti vacuolar protein sorting 41 (yeast homolog) antibody, anti vacuolar protein sorting 41 homolog (S. cerevisiae) antibody, anti HVPS41 antibody, anti hVps41p antibody, anti vacuolar protein sorting 41 antibody

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VPS41 Antibody

Immunohistochemical staining of Human Kidney using VPS41 antibody

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

VPS41 Antibody (orb19098)

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100 μg
$ 530.00
DispatchUsually dispatched within 5-10 working days
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