von Willebrand Factor Antibody / vWF

• Catalog Number: orb749397
• Category: Antibodies
• Description: von Willebrand Factor (vWF) is a glycoprotein produced by bone marrow cells and endothelial cells lining the inside surface of blood vessels. Its primary function is platelet adhesion, binding to Factor VIII, collagen and platelets, to coagulate blood at the site of wounding. The enzyme VWFCP, or vWF-cleaving protease, facilitates clotting by cutting the protein into subunits, increasing its binding capacity.Deficiency or dysfunction of the protein increases the tendency of wounds to bleed, or to bleed more. Over 300 gene mutations have been identified and classified into three types. Type 1 von Willebrand Factor disease is characterized by reduced amounts in the bloodstream, Type 2 by reduced binding ability and Type 3 by a nonfunctional protein.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A recombinant human protein fragment was used as the immunogen for this von Willebrand Factor antibody.
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Application notes: Variations in protocols, secondaries and substrates may require the von Willebrand Factor antibody to be titered for optimal performance.1. FFPE staining requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
• Antibody Type: Primary Antibody
• Clone Number: VWF635
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This von Willebrand Factor antibody is available for research use only.
• Note: For research use only

IHC staining of human tonsil with von Willebrand Factor antibody (clone VWF635).

IHC staining of human bone marrow with von Willebrand Factor antibody (clone VWF635).

SDS-PAGE analysis of purified, BSA-free von Willebrand Factor antibody (clone VWF635) as confirmation of integrity and purity.