ADAMTS13 Mouse Monoclonal Antibody

SKU: orb2960461

Description

Anti-Human ADAMTS13 Antibody (B5J1) is a mouse monoclonal antibody detecting A disintegrin and metalloproteinase with thrombospondin m... in Blocking, ELISA, FCM, SPR. Suitable for Human. Highlights Multi-Application: Validated across multiple applications. Flow Cytometry: Validated for flow cytometry applications.

Research Area

Cardiovascular Research

Images & Validation

• Tested Applications: Blocking, ELISA, FC, SBR
• Reactivity: Human

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a, lambda
• Clone No.: B5J1
• Purity: >95% as determined by SDS-PAGE.
• Purification: Protein A/G purified from cell culture supernatant.
• Endotoxin: Please contact with the lab for this information.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M PBS, pH 7.4.
• Concentration: Lot dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

A disintegrin and metalloproteinase with thrombospondin motifs 13, ADAM-TS 13, ADAM-TS13, ADAMTS-13, 3.4.24.87, von Willebrand factor-cleaving protease, vWF-CP, vWF-cleaving protease, ADAMTS13, C9orf8, UNQ6102/PRO20085

Flow-cytometry using anti-human ADAMTS13 antibody. ADAMTS13 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human ADAMTS13 antibody monoclonal antibody (Catalog # orb2960461, Green Histogram) at a concentration of 5 µg/mL for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human ADAMTS13 antibody. HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human ADAMTS13 antibody monoclonal antibody (Catalog # orb2960461, Green Histogram) at a concentration of 5 µg/mL for 30 mins at RT. After washing, bound antibody was detected using a FITC- conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using PE anti-human ADAMTS13 antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human ADAMTS13 monoclonal antibody (Catalog: orb2960461, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE for Anti-Human ADAMTS13 Antibody.

Quick Database Links

UniProt

Q76LX8