Vimentin (Mesenchymal Cell Marker) Antibody

• Catalog Number: orb388775
• Category: Antibodies
• Description: Mouse monoclonal antibody to VIM
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: VM452
• Tested applications: FC, IHC, WB
• Reactivity: Bovine, Canine, Feline, Gallus, Goat, Human, Porcine
• Isotype: IgG1
• Immunogen: Recombinant full-length human vimentin protein
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Dilution range: FACS: 0.5-1 μg/million cells, IF/ICC: 1-2 μg/ml, WB: 0.25-0.5 μg/ml, IHC-P: 0.25-0.5 μg/ml
• Form/Appearance: 200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: 57-60kDa
• Target: VIM
• Entrez: 7431
• UniProt ID: P08670
• Expression System: CHO cells
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Alternative names: VIM
• Note: For research use only
• Application notes: Vimentin (Mesenchymal Cell Marker)
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Raji Cell Lysate using VIM antibody

Immunohistochemical staining of human Melanoma tissue using VIM antibody

Flow cytometric analysis of Jurkat Cells using VIM antibody