Vimentin (Mesenchymal Cell Marker) Antibody

SKU: orb388775

Description

Mouse monoclonal antibody to VIM

Images & Validation

• Tested Applications: FC, IHC, WB
• Dilution range: FACS: 0.5-1 μg/million cells, IF/ICC: 1-2 μg/ml, WB: 0.25-0.5 μg/ml, IHC-P: 0.25-0.5 μg/ml
• Reactivity: Bovine, Canine, Feline, Gallus, Goat, Human, Porcine
• Application Notes:
  Vimentin (Mesenchymal Cell Marker)

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: VM452
• Immunogen: Recombinant full-length human vimentin protein
• Target: VIM
• Source/Expression System: CHO cells
• Molecular Weight: 57-60kDa

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Disclaimer: For research use only

Alternative Names

VIM

Western blot analysis of Raji Cell Lysate using VIM antibody

Immunohistochemical staining of human Melanoma tissue using VIM antibody

Flow cytometric analysis of Jurkat Cells using VIM antibody

Quick Database Links

Gene Symbol

VIM

UniProt

P08670