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Item 1 of 4
Item 1 of 4
Vimentin (4F8) Mouse mAb
Catalog Number: orb1562972
| Catalog Number | orb1562972 |
|---|---|
| Category | Antibodies |
| Description | Vimentin (4F8) Mouse mAb |
| Target | VIM |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Form/Appearance | Liquid |
| Concentration | 2 mg/ml |
| Buffer/Preservatives | Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3. |
| Purification | Affinity Purified |
| Immunogen | Purified recombinant human Vimentin protein fragments expressed in E.coli. |
| UniProt ID | P08670 |
| MW | Calculated MW: 54 kDa; Observed MW: 57 kDa |
| Tested applications | ICC, IF, IHC-Fr, IHC-P, IP, WB |
| Dilution range | WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 |
| Antibody Type | Primary Antibody |
| Clone Number | 4F8-A5-H5 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | VIM; Vimentin |
| Note | For research use only |
Western blot analysis of Vimentin in Molt4, K562, COS7, Jurkat, Hela and Vimentin negative (Daudi, Ramos, Raji) lysates using Vimentin antibody.
Immunocytochemistry analysis of Vimentin (4F8) in Hela using Vimentin antibody.
Immunoprecipitation analysis of Vimentin (4F8) in Hela lysates using Vimentin antibody.
Immunohistochemistry analysis of paraffin-embedded Human tonsils using Vimentin (4F8) antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
