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Validated
Description
Images & Validation
−| Tested Applications | IF, IHC, KO/KD Validated, WB |
|---|---|
| Dilution Range | WB 1:200 - 1:1000, IHC 1:150 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone No. | BQVK7F9 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 210-466 of human VIM (NP_003371) produced in E.coli. |
| Target | Vimentin |
| Molecular Weight | 53.5 kDa |
| Purification | Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS (pH 7.3) containing 1% rAlbumin, 50% glycerol and 0.02% sodium azide. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Western blot analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VIM ( Right lane) using Vimentin (VIM) antibody
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VIM Antibody (orb1318823)
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