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VCAM1 Antibody
Description
Images & Validation
−| Tested Applications | ELISA, WB |
|---|---|
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Produced from sera of rabbits immunized with highly pure recombinant Human VCAM-1. Human VCAM-1 specific antibody was purified by affinity chromatography employing an immobilized Human VCAM-1 matrix. |
| Target | VCAM1 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−VCAM1 Rabbit Polyclonal Antibody [orb348962]
IHC-P, WB
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
100 μgDog Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA Kit [orb781266]
Canine
3.13-200 ng/mL
1.11 ng/mL
96 T, 48 THuman Vascular Cell Adhesion Molecule 1 (VCAM1) EasyStep ELISA Kit [orb1950017]
Human
1.25-80 ng/mL
0.87 ng/mL
48 T, 96 T

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To detect hVCAM-1 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human VCAM-1 (orb1272626) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVCAM-1.

To detect hVCAM-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVCAM-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

To detect hVCAM-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVCAM-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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Protocol Information
VCAM1 Antibody (orb1272637)
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