VCAM1 Rabbit Polyclonal Antibody

• Catalog Number: orb11550
• Category: Antibodies
• Description: VCAM1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Predicted Reactivity: Rat
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: Recombinant Mouse VCAM 1 protein
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:200-1000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 81 kDa
• Target: VCAM1
• RRID: AB_10746744
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: VCAM1; CD 106; CD106; CD106 Antigen; DKFZp779G2333
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Cao, Wei et al. Reducing-Autophagy Derived Mitochondrial Dysfunction during Resveratrol Promotes Fibroblast-Like Synovial Cell Apoptosis Anat Rec (Hoboken), 301, 1179-1188 (2018)

PubMed: 29461680

Blank control: Mouse Spleen (blue). Primary Antibody: Rabbit Anti-VCAM-1 antibody (orb11550, Green), Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol, The cells were fixed with 2% paraformaldehyde for 10 min at 37°C. Primary antibody (orb11550, 1 µg/8x10^5 cells) were incubated for 30 min at room temperature, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (1 hour) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/FITC antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1: Mouse Kidney tissue lysates, Lane 2: Mouse Spleen tissue lysates, Lane 3: Mouse Cerebrum tissue lysates, Primary: Anti-VCAM1 (orb11550) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 81 kDa, Observed band size: 100 kDa.

Sample: Lane 1: Siha (Human) Cell Lysate at 30 ug, Lane 2: Huvec (Human) Cell Lysate at 30 ug, Lane 3: U251 (Human) Cell Lysate at 30 ug, Primary: Anti-VCAM-1 (orb11550) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 110 kD, Observed band size: 110 kD.

Sample: VCAM1 protein (Human) at 100 ng, Primary: Anti-VCAM-1 (orb11550) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 81 kD, Observed band size: 81 kD.

Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-VCAM-1 Polyclonal Antibody, Unconjugated (orb11550) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.