UROD Antibody

• Catalog Number: orb1248519
• Category: Antibodies
• Description: UROD Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: KARECVGKTVTLQGN
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 40kDa band observed in lysates of cell line K56 (calculated MW of 40.8kDa according to NP_000365.3). Recommended concentration: 0.1-0.3ug/ml. An additional band of unknown identity was also consistently observed at 28kDa. This band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 40kDa
• Target: UROD
• UniProt ID: P06132
• NCBI: NP_000365.3
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: UROD, uroporphyrinogen decarboxylase, PCT, UPD, OT
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 40kDa band observed in lysates of cell line K56 (calculated MW of 40.8kDa according to NP_000365.3). Recommended concentration: 0.1-0.3ug/ml. An additional band of unknown identity was also consistently observed at 28kDa. This band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248519 (0.1 ug/ml) staining of K562 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248519 (5 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.