UCP1 Antibody

SKU: orb1246815

Description

UCP1 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Rat
• Predicted Reactivity: Canine, Human, Mouse
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 35kDa band observed in Rat Adipose tissue and Skeletal Muscle lysates (calculated MW of 33.2kDa according to Rat NP_036814.1). Recommended concentration: 0.5-1 µg/mL. . Primary incubation 1 hour at room temperature.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-EQLKRELSKSRQ
• Target: UCP1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

UCP1, uncoupling protein 1 (mitochondrial, proton carrier), HGNC:12517, SLC25A7, UCP, mitochondrial brown fat uncoupling protein, uncoupling protein 1

orb1246815 (1 ug/ml) staining of Rat Adipose (A) and (0.5 ug/ml) Skeletal Muscle (B) lysate (35 ug protein in RIPA buffer) Detected by chemiluminescence.

Quick Database Links

Gene Symbol

UCP1

UniProt

P25874

RefSeq (Protein):NP_068605.1