Ubiquitin Antibody: Biotin

• Catalog Number: orb413632
• Category: Antibodies
• Description: Rabbit Recombinant monoclonal antibody against Ubiquitin conjugated to Biotin
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: RGL3R
• Tested applications: ELISA, ICC, IF, IHC, WB
• Reactivity: All
• Isotype: IgG
• Immunogen: Ubiquitin conjugated lysozyme
• Antibody Type: Recombinant Antibody
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000); ELISA (1:1000)
• Conjugation: Biotin
• Target: Ubiquitin
• Entrez: 7314
• UniProt ID: P0CG48, P0CG47
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
• Alternative names: Polyubiquitin B antibody, RPS27A antibody, UBA52 a
• Note: For research use only
• Application notes: A 1:1000 dilution of SMC-548 was sufficient for detection of Ubiquitin in 2 µg of purified poly-ubiquitin chains (Poly-Ubiquitin: Ub1-Ub7 WT Chains K63-linked, k48-linked) by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of Purified poly-ubiquitin chains showing detection of Multiple Ubiquitin protein using Rabbit Anti-Ubiquitin Monoclonal Antibody, Clone RGL3R. Lane 1: Molecular Weight Ladder (MW). Lane 2: K63 Poly Ubiquitin. Lane 3: K48 Poly Ubiquitin. Load: 2 μg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Ubiquitin Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG:HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: Multiple.