ATG7 Antibody: Biotin

• Catalog Number: orb55133
• Category: Antibodies
• Description: Rabbit polyclonal antibody against ATG7 conjugated to Biotin.
• Target: ATG7
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Biotin
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
• Purification: Peptide Affinity Purified
• Immunogen: Synthetic peptide from the N-terminal of Human ATG7
• UniProt ID: O95352
• MW: 77.9 kDa
• Tested applications: ELISA, ICC, IF, IHC, WB
• Dilution range: WB (1:1000); ICC/IF (1:100); IHC (1:50)
• Application notes: A 1:1000 dilution of SPC-610 was sufficient for detection of ATG7 in 15 µg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
• Storage: Conjugated antibodies should be stored according to the product label
• Alternative names: Autophagy 7S. cerevisiae, homolog of antibody, APG
• Note: For research use only
• Entrez: 10533
• NCBI: NP_001129503.2

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG7 Polyclonal Antibody. Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG7 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Preautophagosomal Structure, Organelle membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ATG7 Antibody. (D) Composite.

Immunohistochemistry analysis using Rabbit Anti-ATG10 Polyclonal Antibody. Tissue: Brain. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-ATG10 Polyclonal Antibody at 1:50 for 31 min at RT. Counterstain: Hematoxylin. Magnification: 10X.

Western blot analysis of Rat brain cell lysates showing detection of ~77.9 kDa ATG7 protein using Rabbit Anti-ATG7 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat brain cell lysates. Load: 20 μg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG7 Polyclonal Antibody at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~77.9 kDa.

Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~77.9 kDa ATG7 protein using Rabbit Anti-ATG7 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Human Cervical cancer cell line (HeLa) lysate. Load: 15 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG7 Polyclonal Antibody at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~77.9 kDa.