UBE2C Antibody

SKU: orb1248769

Description

UBE2C Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 19kDa band observed in lysates of cell lines HEK293 and HeLa (calculated MW of 19.7kDa according to NP_008950.1). Recommended concentration: 1-3µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-SGDKGISAFPESDN
• Target: UBE2C
• Molecular Weight: Approx 19 kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

UBE2C, ubiquitin-conjugating enzyme E2C, UBCH10, dJ447F3.2, OTTHUMP00000031653, OTTHUMP00000031655, cyclin-selective ubiquitin carrier protein, mitotic-specific ubiquitin-conjugating enzyme, ubiquitin carrier protein E2-C, ubiquitin-protein ligase C

orb1248769 (1 ug/ml) staining of HEK293 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

UBE2C

UniProt

O00762

RefSeq (Protein):NP_008950.1, NP_861515.1, NP_861517.1