Goat anti-UBE2C / UBCH10 Antibody

SKU: orb18433

Description

Goat polyclonal antibody to UBE2C

Images & Validation

• Tested Applications: ELISA, FC, IF, IP, WB
• Dilution range: ELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 10ug/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approximately 20KDa band was detected in mitotic (i.e Nocodazole arrested) HeLa whole cell lysates Recommended for use at 1-3 μg/ml. Immunoprecipitation: 20KDa band precipitated from mitotic HeLa whole cell lysates using protein-G dynabeads. Immunofluorescence: Stained mitotic and late G2 Hela cells, with very faint or no signal in G1 and S phase cels (fixed using paraformaldehyde, primary concentration 2.5 μg/ml, using FITC anti-Goat)

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: UBE2C / UBCH10
• Molecular Weight: 19.7; 17.4; 16.7; 15.8; 14.3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti UBE2C antibody, anti UBCH10 antibody, anti dJ447F3.2 antibody, anti ubiquitin-conjugating enzyme E2C antibody, anti ubiquitin-protein ligase C antibody, anti ubiquitin carrier protein E2-C antibody, anti cyclin-selective ubiquitin carrier protein antibody, anti mitotic-specific ubiquitin-conjugating enzyme antibody, anti RP3-447F3.1 antibody

Immunofluorescence analysis of U2OS cells using UBE2C antibody.

Immunofluorescence analysis of MCF7 cells using UBE2C antibody.

Western blot analysis of HEK293 (A) and HeLa (B) cell lysate using UBE2C antibody.

Flow cytometric analysis of HeLa cells using UBE2C antibody.

Quick Database Links

Gene Symbol

UBE2C / UBCH10

NCBI Gene ID

11065