Tyrosine Kinase Receptor A (TrkA) Antibody

SKU: orb3167444

Description

Our Anti-Tyrosine Kinase Receptor A (TrkA) mouse monoclonal primary antibody detects human, mouse, other mammals (predicted), and rat Tyrosine Kinase Receptor A (TrkA), and is IgG. It is validated for use in ICC, WB.

Images & Validation

• Tested Applications: ICC, WB
• Dilution Range: WB: 1-3 µg/mL; ICC: 1-2 µg/mL
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mammal
• Application Notes:
  WB: Western blotting: 1-3 µg/mL, antibody is suitable for reduced (with DTT or beta-mercaptoethanol) and non-reduced samples. Denatured but not reduced samples provides cleaner blot signals as demonstrated in images. Immunocytochemistry: 1-2 µg/mL. Antibody works on 4% formaldehyde-fixed cells. Note that cells require a permeabilization step, because the antibody detects a cytoplasmic epitope of TrkA. Other applications not yet tested.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Clone No.: BS292
• Immunogen: A synthetic peptide from the intracellular cytoplasmic domain of human TrkA (C-HGPDAKLLAGGE, aa: 604-615) has been used as the immunogen.
• Target: Tyrosine Kinase Receptor A (TrkA)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Lyophilized from PBS, pH 7.4, containing 3% trehalose without preservatives.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Tropomyosin-related kinase receptor; High affinity nerve growth factor receptor; Neurotrophic tyrosine kinase receptor type 1; TRK1 transforming tyrosine kinase protein; p140-TrkA; Trk-A; NTRK1; TRK

Quick Database Links

Gene Symbol

Tyrosine Kinase Receptor A (TrkA)

UniProt

P04629