Phospho-TrkA (Tyr490) Rabbit Polyclonal Antibody

SKU: orb186433

Description

Phospho-TrkA (Tyr490) Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
• Reactivity: Mouse, Rat
• Predicted Reactivity: Equine, Gallus, Human, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human TrkA isoform 1(790aa) around the phosphorylation site of Tyr490 PQ(p-Y)FS
• Target: NTRK1
• Molecular Weight: 90 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

TrkA(phospho Y490); TrkA(phospho Tyr790); p-TrkA(phospho Y490); NTRK1(phospho Y490); CIPA; High affinity nerve growth factor receptor precursor; MTC; Neurotrophic tyrosine kinase receptor type 1; Ntrk 1; Ntrk1; Oncogene TRK; p140 TrkA; TRK 1; Trk A; TRK; TRK1; TRK1 transforming tyrosine kinase protein; Tyrosine Receptor Kinase A.

Blank control (blue): RSC96 cells (fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody: Rabbit Anti-phospho-TrkA (Tyr490) antibody (orb186433), dilution: 0.2 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-TrkA (Tyr490)) Polyclonal Antibody, Unconjugated (orb186433) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-TrkA (Tyr490)) Polyclonal Antibody, Unconjugated (orb186433) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug, Lane 2: Cerebrum (Rat) Lysate at 40 ug, Primary: Anti-phospho-TrkA (Tyr490) (orb186433) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 130 kD, Observed band size: 130 kD.

Quick Database Links

Gene Symbol

NTRK1

UniProt

P04629