TSC1 Antibody

• Catalog Number: orb1247831
• Category: Antibodies
• Description: TSC1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Canine, Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-QLHIMDYNETHHEH
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 150kDa band observed in Human Brain lysates (calculated MW of 130kDa according to NP_000359.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Miloloza et al Hum Mol Genet. 2000 Jul 22;9(12):1721-7; PMID: 10915759). In transfected HEK293 transiently expressing Human TSC1 a band of approx. 120kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:Paraffin embedded Human Uterus and Liver. Recommended concentration: 3.75µg/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: TSC1
• UniProt ID: Q92574
• NCBI: NP_000359.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: TSC1, hamartin, tuberous sclerosis 1 , HGNC:12362,
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 150kDa band observed in Human Brain lysates (calculated MW of 130kDa according to NP_000359.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Miloloza et al Hum Mol Genet. 2000 Jul 22;9(12):1721-7; PMID: 10915759). In transfected HEK293 transiently expressing Human TSC1 a band of approx. 120kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:Paraffin embedded Human Uterus and Liver. Recommended concentration: 3.75µg/ml.
• Expiration Date: 12 months from date of receipt.

orb1247831 (0.1 μg/ml) staining of Human Brain lysate (35 μg protein in RIPA buffer). Detected by chemiluminescence.

orb1247831 (3.75 μg/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247831 (3.75 μg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.