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Catalog Number | orb19208 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to TSC1 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Canine, Human, Mouse, Rat |
Dilution range | ELISA: 1:128000, WB: 0.1-0.3 μg/ml, IHC-P: 3.75 μg/ml |
Conjugation | Unconjugated |
MW | 130 |
Target | Hamartin / TSC1 (isoform 1) |
Entrez | 7248 |
Protein Sequence | QLHIMDYNETHHEH |
RRID | AB_10748666 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti TSC1 antibody, anti hamartin antibody, anti t Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 150kDa band observed in Human Brain lysates (calculated MW of 130kDa according to NP_000359.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Miloloza et al Hum Mol Genet. 2000 Jul 22;9(12):1721-7; PMID: 10915759). In transfected HEK293 transiently expressing Human TSC1 a band of approx. 120kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-0.3 μg/ml.. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of human Brain lysate using TSC1 antibody
Immunohistochemical staining of Human Uterus using TSC1 antibody
Immunohistochemical staining of Human Liver of TSC1 antibody