Hamartin/TSC1 (isoform 1) Antibody

SKU: orb19208

Description

Goat polyclonal antibody to TSC1

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:128000, WB: 0.1-0.3 μg/ml, IHC-P: 3.75 μg/ml
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 150kDa band observed in Human Brain lysates (calculated MW of 130kDa according to NP_000359.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Miloloza et al Hum Mol Genet. 2000 Jul 22;9(12):1721-7; PMID: 10915759). In transfected HEK293 transiently expressing Human TSC1 a band of approx. 120kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-0.3 μg/ml..

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Hamartin / TSC1 (isoform 1)
• Molecular Weight: 130
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti TSC1 antibody, anti hamartin antibody, anti tuberous sclerosis 1 antibody, anti HGNC:12362 antibody, anti KIAA0243 antibody, anti LAM antibody, anti MGC86987 antibody, anti TSC antibody, anti tuberous sclerosis 1 protein antibody, anti tumor suppressor antibody

Western blot analysis of human Brain lysate using TSC1 antibody

Immunohistochemical staining of Human Uterus using TSC1 antibody

Immunohistochemical staining of Human Liver of TSC1 antibody

Quick Database Links

Gene Symbol

Hamartin / TSC1 (isoform 1)

NCBI Gene ID

7248