TRPV4 Antibody

• Catalog Number: orb1240253
• Category: Antibodies
• Description: TRPV4 Antibody
• Target: TRPV4
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1 mg/mL
• Buffer/Preservatives: TRPV4 antibody is supplied in PBS containing 0.02% sodium azide.
• Purification: TRPV4 antibody is affinity chromatography purified via peptide column.
• Immunogen: TRPV4 antibody was raised against an 18 amino acid peptide near the center of human TRPV4.The immunogen is located within amino acids 380 - 430 of TRPV4.
• UniProt ID: Q9HBA0
• MW: Predicted: 96 kDa Observed: 100 kDa
• Tested applications: ELISA, IF, IHC-P, WB
• Application notes: TRPV4 antibody can be used for detection of TRPV4 by Western blot at 1 μg/ml. Antibody can also be used for Immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 5 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: TRPV4 Antibody: SMAL, VRL2, CMT2C, SPSMA, TRP12, V
• Research Area: Signal Transduction
• Note: For research use only
• NCBI: NP_067638

Western blot analysis of TRPV4 in (A) human testis tissue and (B) SK-N-SH cell lysate with TRPV4 antibody at 1 µg/mL.

Immunohistochemistry of TRPV4 in human lung tissue with TRPV4 antibody at 5 µg/mL.

Immunofluorescence of TRPV4 in human lung tissue with TRPV4 antibody at 20 µg/mL.

Immunofluorescence of TRPV4 in human kidney tissue with TRPV4 antibody at 5 µg/ml. Red: TRPV4 Antibody (orb1240253) Blue: DAPI staining.

Immunohistochemistry of TRPV4 in human kidney tissue with TRPV4 antibody at 2 µg/ml.