TRPC4 Antibody

• Catalog Number: orb1246480
• Category: Antibodies
• Description: TRPC4 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine
• Reactivity: Human, Rat
• Immunogen: The immunogen for this antibody is: C-KHAKEEDSSIDYD
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 110kDa band observed in Rat Brain lysates (calculated MW of 112kDa according to Human NP_057263.1 and to Rat NP_536321.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Placenta and Kidney. Recommended concentration: 4.75ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: TRPC4
• UniProt ID: Q9UBN4
• NCBI: NP_001129427.1, NP_001129428.1, NP_001129430.1, NP_001129429.1, NP_003297.1, NP_057263.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: TRPC4, transient receptor potential cation channel
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 110kDa band observed in Rat Brain lysates (calculated MW of 112kDa according to Human NP_057263.1 and to Rat NP_536321.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Placenta and Kidney. Recommended concentration: 4.75ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1246480 (0.3 ug/ml) staining of Rat Brain lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246480 (4.75 ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1246480 (4.75 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.