TRPC1 Antibody

• Catalog Number: orb1538246
• Category: Antibodies
• Description: TRPC1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, IHC-P
• Reactivity: Bovine, Canine, Equine, Gallus, Human, Monkey, Mouse, Porcine, Rabbit, Rat
• Immunogen: Peptide with sequence C-NLKQKRDENYQK, from the internal region of the protein sequence according to NP_003295.1NP_001238774.1.
• Concentration: 0.5 mg/ml
• Dilution range: IF (10 µg/ml), IHC, IHC-P (3.75 µg/ml), Peptide-ELISA (1:32000)
• Conjugation: Unconjugated
• Target: TRPC1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Alternative names: TRPC1, HTRP-1, TRP1, TRP-1, Trrp1, Transient recep
• Note: For research use only
• Application notes: Further information: Immunohistochemistry: LS-B4119 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B4119 was determined to be 3.75 ug/ml.
• Expiration Date: 12 months from date of receipt.

TRPC1 antibody immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml).

TRPC1 antibody flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton.