TRPC1 Antibody

SKU: orb1538246

Description

TRPC1 Antibody

Images & Validation

• Tested Applications: ELISA, IF, IHC, IHC-P
• Dilution range: IF (10 µg/ml), IHC, IHC-P (3.75 µg/ml), Peptide-ELISA (1:32000)
• Reactivity: Bovine, Canine, Equine, Gallus, Human, Monkey, Mouse, Porcine, Rabbit, Rat
• Application Notes:
  Further information: Immunohistochemistry: LS-B4119 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B4119 was determined to be 3.75 ug/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-NLKQKRDENYQK, from the internal region of the protein sequence according to NP_003295.1NP_001238774.1.
• Target: TRPC1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Concentration: 0.5 mg/ml
• Disclaimer: For research use only

Alternative Names

TRPC1, HTRP-1, TRP1, TRP-1, Trrp1, Transient receptor protein 1

TRPC1 antibody immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml).

TRPC1 antibody flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton.

Quick Database Links

Gene Symbol

TRPC1