TRIP13 Antibody

• Catalog Number: orb1264563
• Category: Antibodies
• Description: TRIP13 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This TRIP13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 64-90 amino acids from the N-terminal region of human TRIP13.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 49 kDa
• Target: TRIP13
• UniProt ID: Q15645
• NCBI: Q15645
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Pachytene checkpoint protein 2 homolog, Human papi
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of TRIP13 Antibody in K562, Hela, 293 cell line lysates (35 ug/lane)

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with TRIP13 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of TRIP13 using rabbit polyclonal TRIP13 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TRIP13 gene.