H3K9me3 Antibody

SKU: orb1258188

Description

H3K9me3 Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ChIP, IF, IHC, IP, WB
• Reactivity: Human, Mouse, Other, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:50 - 1:200CHIP seq: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: A synthetic methylated peptide corresponding to residues surrounding K9 of human histone H3
• Target: H3K9me3
• Molecular Weight: Observed: 17kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

H3F3A, H3t, H3.4, H3/g, H3FT

Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K9 antibody (orb1258188) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K9 antibody (orb1258188).

Immunohistochemistry of paraffin-embedded rat testis using TriMethyl-Histone H3-K9 antibody (orb1258188) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse testis using TriMethyl-Histone H3-K9 antibody (orb1258188) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of 293T cells using TriMethyl-Histone H3-K9 antibody (orb1258188). Blue: DAPI for nuclear staining.

Chromatin immunoprecipitation analysis of extracts of 293T cells, using TriMethyl-Histone H3-K9 antibody (orb1258188) and rabbit IgG. P1 and P2 were located on EBAG9 gene. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Quick Database Links

Gene Symbol

H3K9me3

UniProt

Q16695