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Histone H3 (tri methyl K9) Mouse Monoclonal Antibody

Catalog Number: orb499567

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb499567
CategoryAntibodies
DescriptionHistone H3 (tri methyl K9) Mouse Monoclonal Antibody
Species/HostMouse
ClonalityMonoclonal
Clone Number4C3
Tested applicationsIF, IHC-Fr, IHC-P
Predicted ReactivityMouse, Rat
ReactivityHuman, Mouse, Rat
IsotypeIgG1
ImmunogenKLH conjugated synthesised methylpeptide derived from human Histone H3 around the methylation site of Tri Methyl K9 AR(Tri Methyl-K)ST
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeIHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Form/AppearanceLiquid
ConjugationUnconjugated
MW15 kDa
TargetH3C1
UniProt IDQ71DI3
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesHistone H3(tri methyl Lys9); H3K9me3; H3 histone f
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NoteFor research use only
Expiration Date12 months from date of receipt.
Histone H3 (tri methyl K9) Mouse Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Histone H3 (tri methyl K9)) Monoclonal Antibody, Unconjugated (orb499567) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Histone H3 (tri methyl K9) Mouse Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Histone H3 (tri methyl K9)) Monoclonal Antibody, Unconjugated (orb499567) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Histone H3 (tri methyl K9) Mouse Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Histone H3 (tri methyl K9)) Monoclonal Antibody, Unconjugated (orb499567) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Histone H3 (tri methyl K9) Mouse Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Histone H3 (tri methyl K9)) Monoclonal Antibody, Unconjugated (orb499567) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Histone H3 (tri methyl K9) Mouse Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Rat colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Histone H3 (tri methyl K9)) Monoclonal Antibody, Unconjugated (orb499567) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.