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Item 1 of 9
Item 1 of 9
TriMethyl-Histone H3-K27 pAb
Catalog Number: orb1258185
| Catalog Number | orb1258185 |
|---|---|
| Category | Antibodies |
| Description | TriMethyl-Histone H3-K27 pAb |
| Target | H3K27me3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Other, Rat |
| Form/Appearance | Liquid |
| Concentration | batch dependent |
| Buffer/Preservatives | Liquid |
| Immunogen | A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3 |
| UniProt ID | Q16695 |
| MW | Observed: 17kDa |
| Tested applications | ChIP, IF, IHC, IP, WB |
| Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:20 - 1:100CHIP seq: 1:20 - 1:100 |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | H3F3A, H3t, H3.4, H3/g, H3FT |
| Note | For research use only |
Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K27 antibody (orb1258185) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K27 antibody (orb1258185).
Immunohistochemistry of paraffin-embedded rat ovary using TriMethyl-Histone H3-K27 antibody (orb1258185) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast using TriMethyl-Histone H3-K27 antibody (orb1258185) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using TriMethyl-Histone H3-K27 antibody (orb1258185) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of 293T cells using TriMethyl-Histone H3-K27 antibody (orb1258185). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using TriMethyl-Histone H3-K27 antibody (Red) (orb1258185) and BrdU antibody (Green) (A1482). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using TriMethyl-Histone H3-K27 antibody (orb1258185) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using TriMethyl-Histone H3-K27 antibody (orb1258185) at dilution of 1:100. Blue: DAPI for nuclear staining.
