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TriMethyl-Histone H3-K27 pAb

Catalog Number: orb1258185

DispatchUsually dispatched within 5-10 working days
$ 960.00
Catalog Numberorb1258185
CategoryAntibodies
DescriptionTriMethyl-Histone H3-K27 pAb
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsChIP, IF, IHC, IP, WB
ReactivityHuman, Mouse, Other, Rat
IsotypeIgG
ImmunogenA synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3
Antibody TypePrimary Antibody
Concentrationbatch dependent
Form/AppearanceLiquid
ConjugationUnconjugated
MWObserved: 17kDa
TargetH3K27me3
UniProt IDQ16695
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid
Alternative namesH3F3A, H3t, H3.4, H3/g, H3FT
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NoteFor research use only
Application notesWB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:20 - 1:100CHIP seq: 1:20 - 1:100
Expiration Date12 months from date of receipt.
TriMethyl-Histone H3-K27 pAb

Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K27 antibody (orb1258185) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

TriMethyl-Histone H3-K27 pAb

Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K27 antibody (orb1258185).

TriMethyl-Histone H3-K27 pAb

Immunohistochemistry of paraffin-embedded rat ovary using TriMethyl-Histone H3-K27 antibody (orb1258185) at dilution of 1:100 (40x lens).

TriMethyl-Histone H3-K27 pAb

Immunohistochemistry of paraffin-embedded human breast using TriMethyl-Histone H3-K27 antibody (orb1258185) at dilution of 1:100 (40x lens).

TriMethyl-Histone H3-K27 pAb

Immunohistochemistry of paraffin-embedded mouse kidney using TriMethyl-Histone H3-K27 antibody (orb1258185) at dilution of 1:100 (40x lens).

TriMethyl-Histone H3-K27 pAb

Immunofluorescence analysis of 293T cells using TriMethyl-Histone H3-K27 antibody (orb1258185). Blue: DAPI for nuclear staining.

TriMethyl-Histone H3-K27 pAb

Immunofluorescence analysis of HeLa cells using TriMethyl-Histone H3-K27 antibody (Red) (orb1258185) and BrdU antibody (Green) (A1482). Blue: DAPI for nuclear staining.

TriMethyl-Histone H3-K27 pAb

Immunofluorescence analysis of HeLa cells using TriMethyl-Histone H3-K27 antibody (orb1258185) at dilution of 1:100. Blue: DAPI for nuclear staining.

TriMethyl-Histone H3-K27 pAb

Immunofluorescence analysis of C6 cells using TriMethyl-Histone H3-K27 antibody (orb1258185) at dilution of 1:100. Blue: DAPI for nuclear staining.