Tri-Methyl-Histone H3 (Lys27) Recombinant Mouse Monoclonal Antibody

• Catalog Number: orb1173102
• Category: Antibodies
• Description: Tri-Methyl-Histone H3 (Lys27) Recombinant Mouse Monoclonal Antibody
• Species/Host: Mouse
• Clonality: Recombinant
• Clone Number: H8G3
• Tested applications: ICC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Isotype: IgG
• Antibody Type: Recombinant Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-1:2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-1:100, IF=0
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: H3C1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Mouse IgG H&L (HRP), Lysate: HeLa, N2a, BRL, Mouse liver, Mouse kidney, Protein loading quantity: 20 µg, Exposure time: 30 s, Predicted MW: 15 kDa, Observed MW: 15 kDa.

Tissue: Human neuroblastoma, Section type: Formalin fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:200, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Mouse), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1173102.