TRIM71 Antibody

• Catalog Number: orb1249149
• Category: Antibodies
• Description: TRIM71 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine, Rat
• Reactivity: Human, Mouse
• Immunogen: The immunogen for this antibody is: C-RQVKAKSLYLQVE
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 100kDa band observed in lysates of the cell lines HEK293 and NIH3T3 (calculated MW of 93.4kDa according to Human NP_001034200.1 and 92.1kDa according to Mouse NP_001035968.1). The observed molecular weight corresponds to findings with EB09902, a product of different design reacting to a different part of the same protein. Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 3.75ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: TRIM71
• UniProt ID: Q2Q1W2
• NCBI: NP_001034200.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: TRIM71, tripartite motif-containing 71, LIN-41, LI
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 100kDa band observed in lysates of the cell lines HEK293 and NIH3T3 (calculated MW of 93.4kDa according to Human NP_001034200.1 and 92.1kDa according to Mouse NP_001035968.1). The observed molecular weight corresponds to findings with EB09902, a product of different design reacting to a different part of the same protein. Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 3.75ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1249149 (0.3 ug/ml) staining of HEK293 (A) and NIH3T3 (B) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1249149 (3.75 ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.