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| Catalog Number | orb20530 |
|---|---|
| Category | Antibodies |
| Description | Goat polyclonal antibody to TRIM71 |
| Target | TRIM71 (aa390-402) |
| Clonality | Polyclonal |
| Species/Host | Goat |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Bovine, Canine, Rat |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Protein Sequence | RQVKAKSLYLQVE |
| RRID | AB_10933959 |
| MW | 93.4 |
| Tested applications | ELISA, IHC, WB |
| Dilution range | ELISA: 1:8000, WB: 0.3-1 μg/ml, IHC-P: 3.75 μg/ml |
| Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 100kDa band observed in lysates of the cell lines HEK293 and NIH3T3 (calculated MW of 93.4kDa according to NP_001034200.1). The observed molecular weight corresponds to findings with orb20522, a product of different design reacting to a different part of the same protein. Recommended concentration: 0.5-1 μg/ml. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | anti abnormal cell LINeage LIN-41 antibody, anti h Read more... |
| Note | For research use only |
| Entrez | 131405 |

Western blot analysis of HEK293 lysate using TRIM71 antibody.

Western blot analysis of HEK293 (Lane1) and NIH3T3 (Lane2) lysate using TRIM71 antibody

Immunohistochemical staining of Human Cerebellum using TRIM71 antibody
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