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Catalog Number | orb1257254 |
---|---|
Category | Antibodies |
Description | TRIM69 Antibody |
Target | TRIM69 |
Clonality | Polyclonal |
Isotype | IgG |
Conjugation | Unconjugated |
Reactivity | Human, Mouse, Rat |
Form/Appearance | Liquid |
Concentration | batch dependent |
Buffer/Preservatives | Liquid |
Purification | Affinity purification |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-341 of human TRIM69 (NP_542783.2). |
UniProt ID | Q86WT6 |
MW | Observed: 57kDa |
Tested applications | IF, IHC, WB |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Antibody Type | Primary Antibody |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | RNF36, Trif, HSD34, RNF36 |
Note | For research use only |
Immunohistochemistry of paraffin-embedded human kidney using TRIM69 antibody (orb1257254) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of mouse liver, using TRIM69 antibody (orb1257254) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
Immunofluorescence analysis of A549 cells using TRIM69 antibody (orb1257254). Blue: DAPI for nuclear staining.
ELISA, IHC, WB | |
Bovine, Canine, Human, Mouse, Porcine, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
IF, IHC-Fr, IHC-P, WB | |
Bovine, Canine, Human, Sheep | |
Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |