RNF36/TRIM69 Antibody

SKU: orb18468

Description

Goat polyclonal antibody to TRIM69

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:32000, WB: 0.5-2 μg/ml, IHC-P: 2.5 μg/ml
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Porcine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx 20-25kDa band in Human Testis lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 57.4kDa according to NP_892030.3 and of 39.3kDa according to NP_542783.2. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: RNF36 / TRIM69
• Molecular Weight: 57.4; 39.3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti TRIM69 antibody, anti tripartite motif-containing 69 antibody, anti HSD34 antibody, anti RNF36 antibody, anti Trif antibody, anti ring finger protein 36 antibody, anti trimless antibody, anti tripartite motif protein 69 antibody, anti hCG_39321 antibody, anti RNF36 antibody

Immunohistochemical staining of Human Testis using TRIM69 antibody

Western blot analysis of Mouse Testis lysate using TRIM69 antibody

Quick Database Links

Gene Symbol

RNF36 / TRIM69

NCBI Gene ID

140691