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TRIM5 Antibody

SKU: orb1247877

Description

TRIM5 Antibody

Research Area

Signal Transduction

Images & Validation

Tested ApplicationsELISA, WB
ReactivityHuman
Application Notes
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 38kDa band observed in Human Plancenta lysates (calculated MW of 37.7kDa according to NP_149084.1). Recommended concentration: 2-6µg/mL. Primary incubation was 1 hour.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: ASGILVNVKEEVTC
TargetTRIM5
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

TRIM5, RNF88, tripartite motif-containing 5, TRIM5alpha, tripartite motif protein 5, tripartite motif protein TRIM5, OTTHUMP00000069812, tripartite motif protein TRIM, tripartite motif-containing protein 5 alpha

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TRIM5 Antibody

orb1247877 (2 ug/ml) staining of Human Placenta lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

TRIM5 Antibody

orb1247877 (5 ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Documents Download

Datasheet
Product Information
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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

TRIM5 Antibody (orb1247877)

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Available Sizes

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0.1 mg
$ 920.00
DispatchUsually dispatched within 5-10 working days
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